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We investigated the effects of aging on the total body mononuclear cell (MNC) composition, distribution of granulocytes, monocytes and lymphocytes, and organ-associated MNCs of mice. We found that when MNCs from the spleen and liver of young (3 mo) and old (18 mo) mice were examined by flow cytometry, age-dependent changes were observed in the cell distribution among the granulocytes, lymphocytes, and monocytes and in the proportion of MNC subsets. Compared with young mice, more monocytes and fewer granulocytes were found in the spleens and livers of the old mice. The proportion of lymphocytes and monocytes in the spleens of old mice were less than those in the spleens of young mice. In contrast, the proportion of monocytes and lymphocytes in the livers of old mice were greater than those in the livers of young mice. The distribution of granulocytes and monocytes in the spleens and livers did not differ between the groups. The results suggest that the total number of granulocytes and lymphocytes increase, while the proportion of monocytes decrease with age in the spleen and liver. The distribution of monocytes and granulocytes in the spleen and liver of old mice is altered to a higher level of granulocytes and a lower level of monocytes than that of young mice. The proportion of lymphocytes is less than that of granulocytes
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